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Home Antibody All anti-MAP1LC3B antibodies

Anti-MAP1LC3B Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324625
  • Rabbit polyclonal LC3 Antibody (APG8B) (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402952) , 20ug Explanation
400ul 325 3-7 Days
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WB(3)
IHC(1)
IF(4)
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Also for MAP1LC3B (NM_022818)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Clone Name IsotypeIgG
Species ReactivityHuman, Rat (Predicted: Bovine) Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000, IF: 1:100, IHC: 1:50~100
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)
Alternative NameATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a
Database LinkNP_073729
Entrez Gene 81631 Human
Entrez Gene 64862 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #TA324625) in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
WB Image
Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #TA324625) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified Pab.
WB Image
Western blot analysis of anti-LC3 (APG8b) in untreated or treated Hela cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
IHC Image
Wild-type (Cln3+/+) or homozygous Cln3Ã?ex7/8 (Cln3Ã?ex7/8/Ã?ex7/8) paraffin-embedded brain sections immunostained for the LC3 protein (Cat. # TA324625 LC3 antibody). Shown are the CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Few immunopositive puncta are present in wild-type sections, whereas homozygous Cln3Ã?ex7/8 sections contain clusters of LC3-positive puncta around pyramidal neurons and Purkinje cells (P). MOL, molecular layer; GCL, granule cell layer. Data courtesy of Dr. Susan Cotman, Massachusets General Hospital.
IF Image
Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term) (Cat. #TA324625). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324625 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
IF Image
Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B) (N-term)(Cat. #TA324625). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324625 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
IF Image
Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term)(Cat. #TA324625). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324625 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
IF Image
Fluorescent image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

 

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