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Home Antibody All anti-MAP1LC3B antibodies

Anti-MAP1LC3B Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA324625 Rabbit polyclonal LC3 Antibody (APG8B) (N-term) 400ul $325 3-7 Days
LC402952 MAP1LC3B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(3)
IHC(1)
IF(4)
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Also for MAP1LC3B (NM_022818)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Clone Name IsotypeIgG
Species ReactivityHuman, Rat (Predicted: Bovine) Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000, IF: 1:100, IHC: 1:50~100
Predicted MW Explanation 14688 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 beta (MAP1LC3B)
Alternative NameATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a
Database LinkNP_073729
Entrez Gene 81631 Human
Entrez Gene 64862 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #TA324625) in rat brain lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in membrane fraction (P) but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
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Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #TA324625) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). Human LC3 (APG8b) (arrow) was detected using the purified Pab.
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Western blot analysis of anti-LC3 (APG8b) in untreated or treated Hela cell lysate. Both non-lipidated (arrow, I) and lipidated LC3 (APG8b) (arrow, II) were detected in but pro-LC3 (APG8b) and non-lipidated LC3 ((APG8b) were detected in soluble fraction (S).
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Wild-type (Cln3+/+) or homozygous Cln3 paraffin-embedded brain sections immunostained for the LC3 protein (Cat.# TA324625 LC3 antibody). Shown are the CA2/CA3 region of hippocampus (Hc) and cerebellum (Cb) from 10-month-old mice. Few immunopositive puncta are present in wild-type sections, whereas homozygous Cln3 sections contain clusters of LC3-positive puncta around pyramidal neurons and Purkinje cells (P). MOL, molecular layer; GCL, granule cell layer.
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Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term) (Cat. #TA324625). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324625 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
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Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B) (N-term)(Cat. #TA324625). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324625 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
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Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term)(Cat. #TA324625). U251 cells(right) were treated with Chloroquine (50 μM,16h). TA324625 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).
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IF image of U251 cells stained with LC3 (APG8B) (N-term) antibody. U251 cells were treated with Chloroquine , then incubated with AP1801i LC3 (Isoform B Specific) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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