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Home Antibody All anti-MAP1LC3A antibodies

Anti-MAP1LC3A Antibody 166AT1234

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324327
  • Mouse monoclonal LC3 Antibody (APG8)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403169) , 20ug Explanation
400ul 325 In Stock
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WB(2)
IHC(1)
IF(1)
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Also for MAP1LC3A (NM_032514)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Clone Name166AT1234 IsotypeMouse IgG1 k
Species ReactivityHuman, Mouse, Rat Concentration1.09 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000; IHC: 1:50-100; IF: 1:200
BufferPurified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1
Alternative NameATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Database LinkNP_115903
Function
Related Pathway
Senescence and Autophagy

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of anti-LC3 Mab at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
WB Image
Western blot analysis of anti-LC3 Mab Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
IHC Image
10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# TA324327. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
IF Image
Fluorescent image of U251 cells stained with TA324327 LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 .M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324327 LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 .g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

 

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