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Anti-MAP1LC3A Antibody 166AT1234
Also for MAP1LC3A (NM_032514)
|This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).|
||Mouse IgG1 k
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:1000; IHC: 1:50-100; IF: 1:200
|Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1|
|ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3|
Senescence and Autophagy
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Western blot analysis of anti-LC3 Mab at 8 ug/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
Western blot analysis of anti-LC3 Mab Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
10X( lower panel) and 20X (upper panel) immunohistochemistry images from muscle tissue of a diseased mouse off Dox after 5 weeks on regular food. Several fibers that have autophagic vesicles throughout are visible. Primary antibody used is Cat# TA324327. Data courtesy of Dr. Christy Caudill, Cincinnati Children's Hospital Medical Center.
Fluorescent image of U251 cells stained with TA324327 LC3 (APG8) antibody.U251 cells were treated with Chloroquine (50 .M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324327 LC3 (APG8) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 .g/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.