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Anti-MADD Antibody EPR4919
Also for MADD (NM_001135943)
|A synthetic peptide corresponding to residues at the C-terminus of human MADD was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens MAP-kinase activating death domain (MADD), transcript variant 9|
|DENN; IG20; RAB3GEP|
Entrez Gene 8567 Human
Entrez Gene 228355 Mouse
Entrez Gene 94193 Rat
|MADD is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells (1). |
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Western blot - Anti-DENN antibody [EPR4919]; All lanes : Anti-DENN antibody [EPR4919] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : K562 cell lysate .Lane 4 : NIH 3T3 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution.Predicted band size : 183 kDa.Observed band size : 220 kDa .
Other-Anti-DENN antibody [EPR4919](TA310671); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919]; Immunohistochemical analysis of paraffin embedded Human breast tissue labelling DENN with TA310671 at 1/100.