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Home Antibody All anti-Lefty2 antibodies

Anti-Lefty2 Antibody 7C5G1H6H10

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SKU Description Amount Price Availability*  
TA319606
  • Mouse monoclonal LEFTY A antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418816) , 20ug
100ug 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenA BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Clone Name7C5G1H6H10 Isotype
Species Reactivityhuman Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000, WB: 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note During vertebrate embryogenesis, a left-right axis is established.  Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries.  TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors.   Complexes formed move into the nucleus, where they act as components of a transcriptional complex.  Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling.  Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor.  Lefty also inhibits events downstream from R-Smad phosphorylation.  Lefty provides a repressed state of TGF-beta-responsive genes.  The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans.  Members of the TGF-beta superfamily require processing for their activation.   Cleavage is therefore an essential step for Lefty activation.  Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.

Reference Data

Target NameMus musculus left-right determination factor 2 (Lefty2)
Alternative Name6030463A22Rik; AV214969; Ebaf; Lefta; Leftb
Database LinkNP_796073
Function
Related Pathway

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WB Image
Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 ?g of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

 

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