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Anti-LYN PHOSPHO Antibody EP503Y
|A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 396 of human Lyn was used as immunogen. The antibody will detect Lyn’s phosphorylation on Tyrosine 396.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; FC: 1:50; ICC/IF: Use at an assay dependent concentration; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1|
|JTK8; p53Lyn; p56Lyn|
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Western blot - Lyn antibody [EP503Y]; All lanes : Anti-Lyn (phospho Y396) antibody [EP503Y] at 1/10000 dilution.Lane 1 : HeLa cell lysate, non-treated cells.Lane 2 : HeLa cell lysate, cells treated with Pervanadate.Predicted band size : 61 kDa.Observed band size : 56 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Lyn (phospho Y396) antibody [EP503Y](ab40660); ab40660 (1ug/ml) staining Lyn (phospho Y396) in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cell membrane staining seen in the venous sinus.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Lyn (phospho Y396) antibody [EP503Y]; ICC/IF image of ab40660 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.