Anti-LMAN1 Antibody, clone 1A8

Also for LMAN1 (NM_005570)

Gene Name	Homo sapiens lectin, mannose-binding, 1 (LMAN1)
Synonyms:	ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; MCFD1; MR60
Immunogen	Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 1A8	Isotype	IgG1
Species Reactivity	Human, Dog, Rat, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:200~500, IHC 1:150, IF 1:100, FLOW 1:100
Background	The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq].
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (RC207088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LMAN1 mouse monoclonal antibody. (TA502137)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody. (TA502137)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-LMAN1 mouse monoclonal antibody. (TA502137)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-LMAN1 mouse monoclonal antibody. (TA502137)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (TA502137)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (TA502137)

IF Image

Anti-LMAN1 mouse monoclonal antibody (TA502137) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1(RC207088).

FC Image

HEK293T cells transfected with either RC207088 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LMAN1 antibody(TA502137), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-LMAN1 antibody(TA502137),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

OriGene Antibodies in recent publications

Anti-LMAN1 TrueMAB Antibody clone 1A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens