Anti-LIPG Antibody, clone 8E4

Also for LIPG (NM_006033)

Gene Name	Homo sapiens lipase, endothelial (LIPG)
Synonyms:	EDL; EL; PRO719
Immunogen	Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 8E4	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, IP, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
Background	The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (RC209248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LIPG mouse monoclonal antibody. (TA501048)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIPG mouse monoclonal antibody. (TA501048)

IF Image

Anti-LIPG mouse monoclonal antibody (TA501048) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG(RC209248).

IP Image

Immunoprecipitation(IP) of LIPG by using TrueMab monoclonal anti-LIPG antibodies (Negative control: IP without adding anti-LIPG antibody.). For each experiment, 500ul of DDK tagged LIPG overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LIPG antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

FC Image

HEK293T cells transfected with either RC209248 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIPG antibody(TA501048), and then analyzed by flow cytometry.

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Anti-LIPG TrueMAB Antibody clone 8E4

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