Comparison of the dose–response relationship of radiation-induced apoptosis in the hippocampal dentate gyrus and intestinal crypt of adult mice
Radiat Prot Dosimetry, Mar 2012; 148: 492 - 497.
[LGR5
]
Tracking protein aggregation and mislocalization in cells with flow cytometry
Nature Methods doi:10.1038/nmeth.1930
[HSPA1A]
Histone deacetylase inhibitors prevent the degradation and restore the activity of glucocerebrosidase in Gaucher disease
PNAS, Dec 2011; 108: 21200 - 21205.
[anti-DDK antibody]
WB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100, IP 2ug/500ul
Background
The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq].
Related Pathway
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.
Immunoprecipitation(IP) of LIPG by using TrueMab monoclonal anti-LIPG antibodies (Negative control: IP without adding anti-LIPG antibody.). For each experiment, 500ul of DDK tagged LIPG overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LIPG antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC209248 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIPG antibody(TA501018), and then analyzed by flow cytometry.
Researchers who bought this antibody have also purchased: