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Anti-LIPE Antibody EPR2330
Also for LIPE (NM_005357)
|A phospho-specific peptide corresponding to residues surrounding Serine 855 of human HSL was used as an immunogen. The antibody only detects HSL phosphorylated on Serine 855.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 1,000~5,000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens lipase, hormone-sensitive (LIPE)|
|Hormone-sensitive lipase (HSL) is a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis. It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1). HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the adrenals, ovaries, testes, and macrophages (2, 3). HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development. HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis. The HSL protein levels and enzymatic activity in ITf and STf were reported positively correlated with serum testosterone levels during development (4). |
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Western blot - HSL/LIPE (phospho S855) antibody [EPR2330]; All lanes : Anti-Hormone sensitive lipase (phospho S855) antibody [EPR2330] at 1/5000 dilution.Lane 1 : Human skeletal muscle lysate, untreated.Lane 2 : Human skeletal muscle lysate, treated with LP.Lysates/proteins at 10 µg per lane.Secondary.HRP labeled goat anti-rabbit at 1/1000 dilution.Predicted band size : 117 , 83 kDa.Observed band size : 83 kDa .