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Home Antibody All anti-LIMS1 antibodies

Anti-LIMS1 Antibody EP1944

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307260
  • Rabbit monoclonal antibody against LIMS1(clone EP1944)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC417616) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LIMS1 was used as an immunogen.
Clone NameEP1944 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2
Alternative NamePINCH; PINCH-1; PINCH1
Database LinkNP_004978
FunctionLIM and senescent cell antigen-like domains 1 (LIMS1, PINCH1) is an adaptor protein that contains five LIM domains. The LIM domain contains two zinc finger motifs that may direct protein-protein interactions (1, 2). LIMS1 is thought to be involved in integrin and growth factor signaling. During integrin signaling, LIMS1 interacts with ILK through its LIM domain. LIMS1 also acts as a bridge linking ILK to NCK2, assisting in growth factor signaling pathways. LIMS1 has been linked to coupling surface receptors to downstream signaling molecules involved in the regulation of cell differentiation, cell proliferation, and cell survival (3).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - LIMS1 antibody [EP1944]; All lanes : Anti-LIMS1 antibody [EP1944] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : Human fetal kidney lysate.Lane 5 : Human spleen lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 37 kDa.Observed band size : 35 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LIMS1 antibody [EP1944]; Immunohistochemical staining of paraffin-embedded Human kidney tissue using TA307260 at 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - LIMS1 antibody [EP1944]; Immunofluorescent staining of HeLa cells using TA307260 at 1/100 dilution.

 

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