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Anti-LIMS1 Antibody EP1944
Also for LIMS1 (NM_004987)
|A synthetic peptide corresponding to residues in human LIMS1 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens LIM and senescent cell antigen-like domains 1 (LIMS1), transcript variant 2|
|PINCH; PINCH-1; PINCH1|
|LIM and senescent cell antigen-like domains 1 (LIMS1, PINCH1) is an adaptor protein that contains five LIM domains. The LIM domain contains two zinc finger motifs that may direct protein-protein interactions (1, 2). LIMS1 is thought to be involved in integrin and growth factor signaling. During integrin signaling, LIMS1 interacts with ILK through its LIM domain. LIMS1 also acts as a bridge linking ILK to NCK2, assisting in growth factor signaling pathways. LIMS1 has been linked to coupling surface receptors to downstream signaling molecules involved in the regulation of cell differentiation, cell proliferation, and cell survival (3).|
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Western blot - LIMS1 antibody [EP1944]; All lanes : Anti-LIMS1 antibody [EP1944] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : Human fetal kidney lysate.Lane 5 : Human spleen lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 37 kDa.Observed band size : 35 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - LIMS1 antibody [EP1944]; Immunohistochemical staining of paraffin-embedded Human kidney tissue using TA307260 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - LIMS1 antibody [EP1944]; Immunofluorescent staining of HeLa cells using TA307260 at 1/100 dilution.