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Anti-LIMK1 TRUEMAB Antibody clone 2A11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for LIMK1 (NM_002314)
|Full length human recombinant protein of human LIMK1 (NP_002305) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1|
| There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503122)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503122)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503122)
Anti-LIMK1 mouse monoclonal antibody (TA503122) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1(RC218058).
HEK293T cells transfected with either RC218058 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIMK1 antibody(TA503122), and then analyzed by flow cytometry.