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Home Antibody All anti-LIMK1 antibodies

Anti-LIMK1 TRUEMAB Antibody clone 6B4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503012
  • LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone 6B4
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400838) , 20ug Explanation
100ul 379 In Stock
CF503012
  • Carrier-free (BSA/glycerol-free) LIMK1 mouse monoclonal antibody,clone 6B4
100ug $450 3-4 weeks
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WB(2)
IHC(6)
IF(1)
FC(1)
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Also for LIMK1 (NM_002314)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.
Clone Nameclone 6B4 IsotypeIgG2a
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.72 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
Alternative NameLIMK; LIMK-1
Database LinkNP_002305
Entrez Gene 3984 Human
Entrez Gene 16885 Mouse
Entrez Gene 65172 Rat
Entrez Gene 699494 Monkey
Entrez Gene 489800 Dog
FunctionThere are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011].
Related PathwayProtein KinaseDruggable Genome Axon guidanceFc gamma R-mediated phagocytosisRegulation of actin cytoskeleton

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIMK1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503012)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503012)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503012)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503012)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503012)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503012)
IF Image
Anti-LIMK1 mouse monoclonal antibody (TA503012) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1(RC218058).
FC Image
HEK293T cells transfected with either RC218058 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIMK1 antibody(TA503012), and then analyzed by flow cytometry.

 

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