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Anti-LIMK1 TRUEMAB Antibody Clone OTI3H6
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for LIMK1 (NM_002314)
|Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.|
|Human , Rat , Monkey , Mouse
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1|
Entrez Gene 3984 Human
Entrez Gene 16885 Mouse
Entrez Gene 65172 Rat
Entrez Gene 699494 Monkey
|There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.|
|Protein KinaseDruggable Genome Axon guidanceFc gamma R-mediated phagocytosisRegulation of actin cytoskeleton|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIMK1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502904)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502904)
Anti-LIMK1 mouse monoclonal antibody (TA502904) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1(RC218058).
HEK293T cells transfected with either RC218058 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIMK1 antibody(TA502904), and then analyzed by flow cytometry.