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Anti-LGR6 Antibody EPR6874
Also for LGR6 (NM_001017403)
|A synthetic peptide corresponding to residues in the intracellular domain of human LGR6 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for IP.
|Homo sapiens leucine-rich repeat containing G protein-coupled receptor 6 (LGR6), transcript variant 1|
|LGR6 is a member of the leucine-rich repeat-containing subgroup of the G protein-coupled 7-transmembrane protein superfamily. It is a glycoprotein hormone receptor with a large N-terminal extracellular domain that contains leucine-rich repeats important for the formation of a horseshoe-shaped interaction motif for ligand binding (1). |
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Western blot - Anti-GPCR LGR6 antibody [EPR6874]; All lanes : Anti-GPCR LGR6 antibody [EPR6874] at 1/1000 dilution.Lane 1 : Human uterus lysate.Lane 2 : Human ovary lysate.Lane 3 : HUVEC lysate.Lane 4 : Human adrenal gland lysate.Lane 5 : HeLa lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 104 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR LGR6 antibody [EPR6874]; TA310656, at 1/50 dilution, staining GPCR LGR6 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-GPCR LGR6 antibody [EPR6874]; TA310656, at 1/100 dilution, staining GPCR LGR6 in HUVEC cells by Immunofluorescence.
Flow Cytometry-Anti-GPCR LGR6 antibody [EPR6874](TA310656); Overlay histogram showing LoVo cells stained with TA310656 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.