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Home Antibody All anti-LGR5 antibodies

Anti-LGR5 TRUEMAB Antibody clone 2A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (4) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503316
  • LGR5 mouse monoclonal antibody, clone 2A2
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401213) , 20ug
100ul 379 In Stock
CF503316
  • Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody, clone 2A2
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IP(1)
IHC(6)
IF(2)
FC(1)
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Also for LGR5 (NM_003667)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.
Clone Nameclone 2A2 IsotypeIgG1
Species ReactivityHuman , Mouse Concentration0.73mg/ml
Guaranteed Application *WB, IHC, IF, IP, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1
Alternative NameFEX; GPR49; GPR67; GRP49; HG38
Database LinkNP_003658
Function
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA503316)
IF Image
Anti-LGR5 mouse monoclonal antibody (TA503316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5(RC212825).
IF Image
Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (TA503316, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).
IP Image
Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC212825 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody(TA503316), and then analyzed by flow cytometry.

 

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