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 Home All anti-LGR5 antibodies

Anti-LGR5 TrueMAB Antibody clone 2A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA503316
  • Purified LGR5 mouse monoclonal antibody, clone 2A2
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401213) , 20ug
 100ul $379 In Stock Add to Shopping Cart
CF503316
  • Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody, clone 2A2
100ug $450 8-10 Days Add to Shopping Cart
WB(1)
IP(1)
IHC(6)
IF(1)
FC(1)
Gene NameHomo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1
Synonyms:FEX; GPR49; GPR67; GRP49; HG38
ImmunogenHuman recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 2A2 IsotypeIgG1
Species ReactivityHuman , Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, IP, FC Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2ug/500ul
Background
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-LGR5 mouse monoclonal antibody. (TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (TA503316)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-LGR5 mouse monoclonal antibody. (TA503316)
IF Image
Anti-LGR5 mouse monoclonal antibody (TA503316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5(RC212825).
IP Image
Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
HEK293T cells transfected with either RC212825 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGR5 antibody(TA503316), and then analyzed by flow cytometry.

 

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