Home Antibody All anti-LGR5 antibodies
Also for LGR5 (NM_003667)
|This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49.|
|WB, IHC, IF, FC
||WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|Homo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1|
|FEX; GPR49; GPR67; GRP49; HG38|
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LGR5/GPR49 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of LGR5/GPR49 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.