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Anti-LGR5 Antibody EPR3065Y
Also for LGR5 (NM_003667)
|A synthetic peptide corresponding to residues in human GPR49 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||FC: 1:1000; WB: 1:1000; IP: 1:20; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC/IF.
|Homo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1|
|FEX; GPR49; GPR67; GRP49; HG38|
|G-protein coupled receptor GPR49 (also known as Leucine-rich repeat containing G-protein coupled receptor 5, Lgr5) is closely related to members of the glycoprotein hormone receptor subfamily. It is expressed in skeletal muscle, placenta, spinal cord, and various regions of the brain (1). GPR49 is reported to possibly mark stem cells in multiple adult tissues and is over expressed in cancers (2). |
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Western blot - GPCR GPR49 antibody [EPR3065Y]; Anti-GPCR GPR49 antibody [EPR3065Y] at 1/1000 dilution + Fetal skeletal muscle lysate at 10 µg.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 100 kDa.Observed band size : 100 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GPCR GPR49 antibody [EPR3065Y]; Panel A: Immunohistochemical analysis of paraffin-embedded human muscle tissue using ab75850 at a dilution of 1/100-1/250. Panel B: Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using ab75850 at a dilution of 1/100-1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR49 antibody [EPR3065Y]; Immunohistochemical analysis of Human colorectal cancer tissue, staining GPCR GPR49 with ab75850. Antigen retrieval was performed by heating in a citrate buffer (pH 6.0) in a microwave oven for 2 min at 100°C. Samples were incubated with primary antibody (1/50 in blocking solution) overnight at 4°C. Staining was detected using DAB.
Flow Cytometry - Anti-GPCR GPR49 antibody [EPR3065Y]; Overlay histogram showing SH-SY5Y cells stained with ab75850 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.