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Home Antibody All anti-LEMD3 antibodies

Anti-LEMD3 TRUEMAB Antibody clone 3C8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500815
  • LEMD3 (MAN1) mouse monoclonal antibody, clone 3C8
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402311) , 20ug Explanation
100ul 325 In Stock
CF500815
  • Carrier-free (BSA/glycerol-free) LEMD3 mouse monoclonal antibody, clone 3C8
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
FC(2)

OriGene Data

ImmunogenFull length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.
Clone Nameclone 3C8 IsotypeIgG2b
Species ReactivityHuman, Monkey Concentration0.83 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:500~2000, FLOW 1:100
Predicted MW Explanation 99.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens LEM domain containing 3 (LEMD3), transcript variant 1
Alternative NameMAN1
Database LinkNP_055134
Entrez Gene 23592 Human
Entrez Gene 717597 Monkey
FunctionThis locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.
Related PathwayTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (RC212759, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.
FC Image
Flow cytometric Analysis of Hela cells, using anti-LEMD3 antibody(TA500815),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-LEMD3 antibody(TA500815),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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