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Anti-LEMD3 TRUEMAB Antibody Clone 3H2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for LEMD3 (NM_014319)
|Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.|
|Human, Dog, Monkey
|WB, IF, IP
||WB 1:500, IF 1:100, IP 2-4ug/mg
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens LEM domain containing 3 (LEMD3), transcript variant 1|
Entrez Gene 23592 Human
Entrez Gene 717597 Monkey
Entrez Gene 608380 Dog
|This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (RC212759, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.
Anti-LEMD3 mouse monoclonal antibody (TA500795) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3(RC212759).
Immunoprecipitation of LEMD3 by using TrueMab monoclonal anti-LEMD3 antibody (Negative control: IP without adding anti-LEMD3 antibody). For each experiment, 500ul of DDK tagged LEMD3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant