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Anti-LEF1 Antibody EPR2029Y
Also for LEF1 (NM_016269)
|A synthetic peptide corresponding to residues in human LEF-1/TCF-1 alpha was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens lymphoid enhancer-binding factor 1 (LEF1), transcript variant 1|
|LEF-1; TCF10; TCF1ALPHA; TCF7L3|
|The human T cell-specific transcription factor LEF-1/TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions (1). TCF-1 alpha participates in the regulation of TCR alpha enhancer by facilitating the assembly of multiple proteins into a higher order nucleoprotein complex. The function of LEF-1/TCF-1 is dependent, in part, on the HMG domain that induces a sharp bend in the DNA helix, and on an activation domain that stimulates transcription only in a specific context of other enhancer-binding proteins (2). Constitutive activation of the Wnt signaling pathway is a root cause of many colon cancers. Activation of this pathway is caused by genetic mutations that stabilize the beta-catenin protein, allowing it to accumulate in the nucleus and form complexes with any member of the lymphoid enhancer factor and T-cell factor family of transcription factors (referred to collectively as LEF/TCFs) to activate transcription of target genes (3). |
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Anti-LEF1 antibody [EPR2029Y]; Anti-LEF1 antibody [EPR2029Y] at 1/1000 dilution + Jurkat cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 44 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 antibody [EPR2029Y]; Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LEF1 with TA301292 at 1/100.