Home Antibody All anti-LDHB antibodies
Anti-LDHB Antibody EP1566Y
Also for LDHB (NM_002300)
|A synthetic peptide corresponding to residues in human LDH-B was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: 1:50 - 1:100; WB: 1:10000; IP: 1:100; FC: 1:50 - 1:80; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1|
|HEL-S-281; LDH-B; LDH-H; LDHBD; TRG-5|
Entrez Gene 3945 Human
Entrez Gene 16832 Mouse
Entrez Gene 24534 Rat
|Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2). |
|Druggable Genome Glycolysis / GluconeogenesisCysteine and methionine metabolismPyruvate metabolismPropanoate metabolismMetabolic pathways|
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Lactate Dehydrogenase antibody [EP1566Y]; Anti-Lactate Dehydrogenase antibody [EP1566Y] at 1/100000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 37 kDa.Observed band size : 37 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Lactate Dehydrogenase antibody [EP1566Y]; Immunohistochemical analysis of paraffin-embedded human liver carcinoma using TA300872 at a 1/50 dilution.
ICC/IF image of TA300872 stained HeLa cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-Lactate Dehydrogenase antibody(TA300872); Overlay histogram showing HeLa cells stained with TA300872 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.