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Anti-LDHA Antibody EP1563Y
Also for LDHA (NM_005566)
|A synthetic peptide corresponding to residues in human LDH-A was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:5000; IP: 1:70; IHC-P: 1:100 - 1:250; FC: 1:30
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1|
|GSD11; LDH1; LDHM; PIG19|
|Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3). |
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Western blot - Anti-Lactate Dehydrogenase antibody [EP1563Y]; Anti-Lactate Dehydrogenase antibody [EP1563Y] at 1/1000 dilution + A549 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 37 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1563Y]; Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma labelling Lactate Dehydrogenase with ab134187 at 1/100 dilution.