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Anti-LDHA Antibody EP1563Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300879 Rabbit Monoclonal Antibody against LDHA (Clone EP1563Y) 100ul $325 In Stock
LC401712 LDHA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human LDH-A was used as immunogen.
Clone NameEP1563Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:5000; IP: 1:70; IHC-P: 1:100 - 1:250; FC: 1:30
Predicted MW Explanation 37 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens lactate dehydrogenase A (LDHA), transcript variant 1
Alternative NameGSD11; HEL-S-133P; LDH1; LDHM; PIG19
Database LinkNP_005557
Entrez Gene 3939 Human
Entrez Gene 16828 Mouse
Entrez Gene 24533 Rat
FunctionLactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
Related PathwayDruggable Genome Glycolysis / GluconeogenesisCysteine and methionine metabolismPyruvate metabolismPropanoate metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Lactate Dehydrogenase antibody [EP1563Y]; Anti-Lactate Dehydrogenase antibody [EP1563Y] at 1/1000 dilution + A549 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 37 kDa.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1563Y]; Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma labelling Lactate Dehydrogenase with TA300879 at 1/100 dilution.


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