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Anti-LCK Antibody Y123
|A synthetic peptide corresponding to residues before SH3 domain of human Lck was used as immunogen. The antibody does not cross-react with other SRC family member.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:100; FC: 1:20; IP: 1:200
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK), transcript variant 2|
|LSK; p56lck; pp58lck; YT16|
|The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4). |
Delta-Notch Signaling Pathway
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Western blot - Lck antibody [Y123]; Anti-Lck antibody [Y123] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lck antibody [Y123]; ab32149 at a 1/250 dilution staining Lck in human lymphoma using Immunohistochemistry, Paraffin Embedded Tissue.
Flow Cytometry-Anti-Lck antibody [Y123](ab32149); Overlay histogram showing Jurkat cells stained with ab32149 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.