Home Antibody All anti-LCK antibodies
Anti-LCK Antibody Y123
|A synthetic peptide corresponding to residues before SH3 domain of human Lck was used as immunogen. The antibody does not cross-react with other SRC family member.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:100; FC: 1:20; IP: 1:200
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Does not react with Mouse, Rat
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Lck antibody [Y123]; Anti-Lck antibody [Y123] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 58 kDa.Observed band size : 58 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Lck antibody [Y123]; TA300455 at a 1/250 dilution staining Lck in human lymphoma using Immunohistochemistry, Paraffin Embedded Tissue.
Flow Cytometry-Anti-Lck antibody [Y123](TA300455); Overlay histogram showing Jurkat cells stained with TA300455 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.