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Anti-LC3A Antibody EPR1754
Also for LC3A (NM_032514)
|A synthetic peptide corresponding to residues on the N-terminus of human LC3A was used as an immunogen|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1,000 - 10,000; ICC: 1:250; FC: 1:40;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A), transcript variant 1|
|ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3|
|Microtubule-associated LC3A constitute nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions (1). Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes (2).|
Senescence and Autophagy
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Western blot - Anti-MAP1LC3A antibody [EPR1754]; Anti-MAP1LC3A antibody [EPR1754] at 1/500 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 14 kDa.