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Home Antibody All anti-LAT antibodies

Anti-LAT Antibody EP983(2)Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301133
  • Rabbit Monoclonal Antibody against LAT (Clone EP983(2)Y)
100ul 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyrosine 200 of human LAT (long isoform) was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.
Clone NameEP983(2)Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:5000; ICC: 1: 100~250
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens linker for activation of T cells (LAT), transcript variant 1
Alternative NameLAT1; pp36
Database LinkNP_055202
FunctionLAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 (on the short isoform of LAT) are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1. Tyr200 and Tyr220 on long isoform of LAT correspond to Tyr171 and Tyr191 on short isoform respectively (3).
Related Pathway

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WB Image
Western blot - LAT (phospho Y200) antibody [EP983(2)Y]; All lanes : Anti-LAT (phospho Y200) antibody [EP983(2)Y] at 1/10000 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate, treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP-labeled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : ~35 kDa .

 

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