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Anti-KSR1 PHOSPHO Antibody EP2358Y
Also for KSR1 (NM_014238)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 404 of human KSR1 was used as immunogen|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens kinase suppressor of ras 1 (KSR1)|
|Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (1). MARK3 phosphorylates serine 309 and serine 404 on KSR1. While, PPP2CA dephosphorlyates serine 404 on KSR1. MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signalling, indicating a link between two important developmental pathways (2). |
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Western blot - KSR1 (phospho S404) antibody [EP2358Y]; All lanes : Anti-KSR1 (phospho S404) antibody [EP2358Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate, treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labelled IgG at 1/1000 dilution.Predicted band size : 102 kDa.Observed band size : 110 kDa .
Immunocytochemistry/ Immunofluorescence - KSR1 (phospho S404) antibody [EP2358Y]; Immunofluorescent staining of Sh-SY-5Y cells, using ab76248, at 1/100 dilution.