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Home Antibody All anti-KRT8 antibodies

Anti-KRT8 TRUEMAB Antibody Clone OTI1B12

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500021 CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12) 100ul $325 In Stock
LC419425 KRT8 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500021 Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone OTI1B12 (formerly 1B12) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(3)
IHC(6)
IF(3)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.
Clone NameClone OTI1B12 IsotypeIgG2b
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.6 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2500~5000, IHC 1:50 - 1:150, IF 1:50 - 1:100
Predicted MW Explanation 53.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens keratin 8, type II (KRT8), transcript variant 2
Alternative NameCARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Database LinkNP_002264
Entrez Gene 3856 Human
Entrez Gene 25626 Rat
Entrez Gene 713118 Monkey
Entrez Gene 486513 Dog
FunctionKeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
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Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-KRT8 monoclonal antibody at 1:1000 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
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Anti-KRT8 mouse monoclonal antibody (TA500021) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
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Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody (TA500021).
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Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500021).
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