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Anti-KRT8 PHOSPHO Antibody EP1629Y
Also for KRT8 (NM_002273)
|A phospho specific peptide corresponding to residues surrounding serine 23 of human CK-8 was used as an immunogen. This antibody detects CK-8 phosphorylated on serine 23.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||IHC-Fr: Use at an assay dependent concentration; IP: 1:10 - 1:100; WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens keratin 8 (KRT8), transcript variant 2|
|CARD2; CK-8; CK8; CYK8; K2C8; K8; KO|
|Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4). |
EGFR1 Signaling Pathway
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Western blot - Cytokeratin 8 (phospho S23) antibody [EP1629Y]; All lanes : Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] at 1/50000 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate treated with Calyculin.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 8 (phospho S23) antibody [EP1629Y]; ab76584, at a 1/100 dilution, staining Cytokeratin 8 in paraffin embedded human gastric adenocarcinoma (image 1) and human breast adenocarcinoma (image 2) tissues by Immunohistochemistry.