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Home Antibody All anti-KRT8 antibodies

Anti-KRT8 Antibody EP1628Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300929
  • Rabbit Monoclonal Antibody against KRT8 (Clone EP1628Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419425) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(7)
IF(1)
Assay(1)
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Also for KRT8 (NM_002273)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human CK8 was used as an immunogen.
Clone NameEP1628Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:25000 - 1:50000; IP: 1:70; FC: 1:20; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 54 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens keratin 8 (KRT8), transcript variant 2
Alternative NameCARD2; CK-8; CK8; CYK8; K2C8; K8; KO
Database LinkNP_002264
Entrez Gene 3856 Human
Entrez Gene 16691 Mouse
FunctionKeratins, the intermediate filament proteins of epithelial cells, connect to desmosomes, the cell-cell adhesion structures at the surface membrane. Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells (1). Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine (2). While K8/K18 are essential for maintaining structural integrity, there is accumulating evidence indicating that they also exert non-mechanical functions. It has been reported that K8/K18-free hepatocytes from K8-null mice are more sensitive to Fas-mediated apoptosis, in line with an increased Fas density at the cell surface and an altered c-Flip regulation of the anti-apoptotic ERK1/2 signaling pathway (3).
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cytokeratin 8 antibody [EP1628Y]; Anti-Cytokeratin 8 antibody [EP1628Y] at 1/50000 dilution + A431 cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 52 kDa .
Assay Image
Other-Anti-Cytokeratin 8 antibody [EP1628Y](TA300929); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y]; Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)Preparation:Fix in 3%PFA in PBS for 30 min at RTIncubate in 7.5% sucrose-PBS for 3h at RTIncubate in 15% sucrose-PBS at 4 degree Celsius overnightEmbed the EBs in tissue-Tek OCT compoundCut frozen sections to 4-20 um thicknessPrimary antibody 1: Rabbit anti cytokeratin 8, 1:100Primary antibody 2: Rat anti-perlecan, 1:100.Secondary antibody 1: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor? 488) pre-adsorbed , 1:200Secondary antibody 2: Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy5?) pre-adsorbed , 1:200.Nuclei were counterstained with DAPI
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 8 antibody [EP1628Y]; Ab53280 (1:250) staining human Cyotkeratin 8 in human breast adenocarcinoma tissue by immunohistochemistry using paraffin embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 8 antibody [EP1628Y](TA300929); Fluorescent immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TA300929. Green-CK8 red-PI.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 8 antibody [EP1628Y](TA300929); Fluorescent immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using TA300929. Green-CK8 red-PI.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 8 antibody [EP1628Y](TA300929); Fluorescent immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue using TA300929. Green-CK8 red-PI.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 8 antibody [EP1628Y](TA300929); Fluorescent immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using TA300929. Green-CK8 red-PI.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y]; TA300929 staining Cytokeratin 8 in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formalin and blocked with 10% serum for 20 minutes at 23°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in TBS + 1% BSA) for 1 hour at 23°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Cytokeratin 8 antibody [EP1628Y]; Immunofluorescent staining of HeLa cells using TA300929 (1:100).

 

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