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Anti-KRT6C Antibody C11
Also for KRT6C (NM_173086)
|This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.|
||Lot dependent; please refer to CoA along with shipment
||ELISA: 1:5,000 - 1:20,000, WB: 1:50 - 1:200, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:100
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial
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Western blot using Immunochemical's Mouse Anti-Keratin antibody. This antibody recognizes a single 56 kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 ng of keratin from human epidermis (Sigma p/n K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
Immunofluorescence Microscopy of Immunochemical's Anti-Keratin antibody (TA319603) was used with Dylight 488 goat anti-mouse 610-141-121 (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, polyclonal Anti-HDAC-1 antibody (TA319251) was used with Atto425 Anti-Rabbit IgG 611-151-122 (shown in red) to detect HDAC-1. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA
Immunofluorescence using Immunochemical's Mouse Anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 µm where the image is taken near the bottom of the cell. Use FITC a 1:2,000 dilution of FITC conjugated Goat-a-Mouse IgG [H&L] (610-102-121) for detection