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Anti-KRT6A Antibody EPR1602Y
Also for KRT6A (NM_005554)
|A synthetic peptide corresponding to residues on the N-terminus of human Cytokeratin 6 was used as an immunogen. This antibody is predicted to detect CK-6a, CK-6b and CK-6c|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||IHC-P: 1:250 - 1:500; WB: 1:5000 - 1:10000; ICC: 1:100 - 1:250; IP: 1:30; FC: 1:100; ICC/IF: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens keratin 6A (KRT6A)|
|CK6A; CK6C; CK6D; K6A; K6C; K6D; KRT6C; KRT6D|
|The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq]. |
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Western blot - Cytokeratin 6 antibody [EPR1602Y]; Anti-Cytokeratin 6 antibody [EPR1602Y] at 1/5000 dilution + A431 cell lysate at 10 µg.Predicted band size : 60 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 6 antibody [EPR1602Y]; Immunohistochemical analysis of paraffin-embedded Human squamous cervical carcinoma using ab93279 at 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1602Y]; ICC/IF image of ab93279 stained A431 cells. The cells were 100%methanol fixed (5 min) and then incubated in 1%BSA / 10% normalgoat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488goat anti-rabiit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-Cytokeratin 6 antibody [EPR1602Y]; Overlay histogram showing A431 cells stained with ab93279 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.