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Anti-KRT5 Antibody EPR1600Y
Also for KRT5 (NM_000424)
|A synthetic peptide corresponding to residues in the head region of human cytokeratin 5 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1: 10,000; IHC: 1: 100; ICC: 1:250; IP: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens keratin 5 (KRT5)|
|CK5; DDD; DDD1; EBS2; K5; KRT5A|
|Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. The mitotically active basal layers of most stratified squamous epithelia express 10 to 30% of their total protein as keratin. The two keratins specifically expressed in these cells are the type II keratin CK-5 and its corresponding partner, type I keratin CK-14, both of which are essential for the formation of 8-nm filaments (1). CK-5 and calretinin have been useful in different studies as immunohistochemical markers suggestive of mesothelioma, and their expression is analyzed for the histological differential diagnosis with adenocarcinomas, especially when confronting with metastatic tumors of unknown origin (2). Loss-of-function mutations in the keratin 5 gene (KRT5) affected family members and in six unrelated patients with Dowling-Degos disease (DDD), an autosomal dominant genodermatosis. This suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3). |
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Western blot - Cytokeratin 5 antibody [EPR1600Y]; Anti-Cytokeratin 5 antibody [EPR1600Y] at 1/10000 dilution + A431 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 62 kDa.Observed band size : 62 kDa.
Other-Anti-Cytokeratin 5 antibody [EPR1600Y](ab75869); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 5 antibody [EPR1600Y]; ab75869 at 1/100 dilution staining Cytokeratin 5 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EPR1600Y](ab75869); Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using ab75869. Green-CK5 red-PI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EPR1600Y]; Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma using ab75869. Green-CK5 red-PI.