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Home Antibody All anti-KRT5 antibodies

Anti-KRT5 Antibody EP1601Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300887 Rabbit Monoclonal Antibody against KRT5 (Clone EP1601Y) 100ul $325 In Stock
LC400149 KRT5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(7)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human CK-5 was used as an immunogen.
Clone NameEP1601Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; ICC/IF: Use at an assay dependent dilution; WB: 1:10000; IHC-P: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens keratin 5, type II (KRT5)
Alternative NameCK5; DDD; DDD1; EBS2; K5; KRT5A
Database LinkNP_000415
Entrez Gene 3852 Human
Entrez Gene 110308 Mouse
FunctionKeratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Cytokeratin 5 antibody [EP1601Y]; Anti-Cytokeratin 5 antibody [EP1601Y] at 1/10000 dilution + A431 cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 62 kDa.Observed band size : 62 kDa.
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Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 5 antibody [EP1601Y]; Human transitional urinary bladder carcinoma stained with TA300887 at 1/100 - 1/250 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EP1601Y](TA300887); TA300887 showing positive staining in Basal cell breast carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EP1601Y](TA300887); TA300887 showing negative staining in Ductal breast carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EP1601Y](TA300887); TA300887 showing positive staining in Squamous cell cervical carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EP1601Y](TA300887); TA300887 showing negative staining in Stomach adenocarcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EP1601Y](TA300887); TA300887 showing positive staining in Normal tonsil squamous cells tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 5 antibody [EP1601Y](TA300887); TA300887 showing positive staining in Squamous cell lung carcinoma tissue.
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Immunocytochemistry/ Immunofluorescence - Cytokeratin 5 antibody [EP1601Y]; A431 cells stained with TA300887 at 1/100 - 1/250
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