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Anti-KRT4 Antibody EP1599Y
Also for KRT4 (NM_002272)
|A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 4 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:50 - 1:100; IP: 1:10 - 1:100; WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens keratin 4 (KRT4)|
|CK-4; CK4; CYK4; K4|
|The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
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Western blot - Cytokeratin 4 antibody [EP1599Y]; Anti-Cytokeratin 4 antibody [EP1599Y] at 1/1000 dilution + A431 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 57 kDa.Observed band size : 59 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 4 antibody [EP1599Y]; Immunohistochemical staining of paraffin embedded human cervical carcinoma tissue using ab51599 (1/100). Goat anti-rabbit HRP (1/2000) used for secondary staining.