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Anti-KRT4 Antibody polyclonal
Also for KRT4 (NM_002272)
|A synthetic peptide corresponding to residues near the N-terminus of human CK-4 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: 1:100 - 1:250; WB: 1:2000; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Does not react with Mouse
|Homo sapiens keratin 4 (KRT4)|
|CK-4; CK4; CYK4; K4|
|The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. |
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Western blot - Cytokeratin 4 antibody; Anti-Cytokeratin 4 antibody at 1/2000 dilution + A431 cell lysate at 10 µg.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 57 kDa.Observed band size : 59 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 4 antibody; Immunohistochemical analysis of paraffin embedded human transitional bladder carcinoma using ab51600 (1/100). Secondary staining with Goat anti-Rabbit HRP (1/2000).