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Home Antibody All anti-KRT20 antibodies

Anti-KRT20 Antibody EPR1622Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300936
  • Rabbit Monoclonal Antibody against KRT20 (Clone EPR1622Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402725) , 20ug Explanation
100ul $325 3-7 Days
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WB(2)
IHC(7)
FC(1)
Assay(1)
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Also for KRT20 (NM_019010)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20 was used as an immunogen.
Clone NameEPR1622Y IsotypeIgG
Species ReactivityRat, Goat, Human, Pig, Marmoset (common) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:10000 - 1:50000; IP: 1:40; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:1000
Predicted MW Explanation 48 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens keratin 20, type I (KRT20)
Alternative NameCD20; CK-20; CK20; K20; KRT21
Database LinkNP_061883
Entrez Gene 54474 Human
Entrez Gene 286912 Rat
FunctionIntermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Cytokeratin 20 antibody [EPR1622Y]; Anti-Cytokeratin 20 antibody [EPR1622Y] at 1/50000 dilution + human small intestine lysate at 10 ug.Secondary.goat anti-rabbit-HRP at 1/1000 dilution.Predicted band size : 48 kDa.Observed band size : 44 kDa .
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Western blot - Anti-Cytokeratin 20 antibody [EPR1622Y]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 48 kDa.
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Other-Anti-Cytokeratin 20 antibody [EPR1622Y](TA300936); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 20 antibody [EPR1622Y]; TA300936 at 1/100 dilution staining Cytokeratin 20 in human colon adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 20 antibody [EPR1622Y]; TA300936 staining Cytokeratin 20 in mouse liver tissue nodules by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with formaldehyde, a heat mediated antigen retrieval step was performed and then the tissue samples were blocked with 10% serum for 30 minutes at 25°C followed by incubation with the primary antibody, at a 1/100 dilution, for 1 hour at 25°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 20 antibody [EPR1622Y](TA300936); TA300936 showing positive staining in Colonic adenocarcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 20 antibody [EPR1622Y](TA300936); TA300936 showing positive staining in Urinary bladder transitional carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 20 antibody [EPR1622Y](TA300936); TA300936 showing negative staining in Hepatocellular carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 20 antibody [EPR1622Y](TA300936); TA300936 showing negative staining in Breast carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 20 antibody [EPR1622Y](TA300936); Fluorescent immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using TA300936. Green-CK20 red-PI.
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Flow Cytometry - Anti-Cytokeratin 20 antibody [EPR1622Y]; Overlay histogram showing LoVo cells stained with TA300936 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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