Home Antibody All anti-KRT18 antibodies
Also for KRT18 (NM_000224)
|A synthetic peptide corresponding to residues on the C-terminus of human CK-18 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IP: 1:100; FC: 1:15; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Immunogen affinity purified (Protein A or G Sepharose)
|Homo sapiens keratin 18 (KRT18), transcript variant 1|
|Cytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3). |
EGFR1 Signaling Pathway
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Western blot - Cytokeratin 18 antibody; Anti-Cytokeratin 18 antibody at 1/50000 dilution + A431 cell lysate at 10 µg.Secondary.goat anti-rabbit HR at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 48 kDa.
Western blot - Cytokeratin 18 antibody; Anti-Cytokeratin 18 antibody at 1/250 dilution + Human Cytokeratin 18 full length protein at 0.01 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 1 minute
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody; ab52948 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody; ab52948 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence