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Home Antibody All anti-KRT18 antibodies

Anti-KRT18 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300825
  • Rabbit Polyclonal Antibody against KRT18
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400083) , 20ug
100ul $379 3-7 Days
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WB(2)
IHC(1)
IF(1)
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Also for KRT18 (NM_000224)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human CK-18 was used as an immunogen.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:100; FC: 1:15; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Immunogen affinity purified

Reference Data

Target NameHomo sapiens keratin 18 (KRT18), transcript variant 1
Alternative NameCYK18; K18
Database LinkNP_000215
FunctionCytokeratin 18 (CK-18), a normal constituent of the hepatocyte cytoskeleton) is a standard tumor marker. CK-18 is elevated in patients with non-malignant liver diseases, particularly in those with prominent cytolysis (1). CK-18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Keratin 8 and 18 (CK8/18) are the major keratin pair in simple-type epithelia, as found in the liver, pancreas, and intestine. Transgenic mice that express mutant keratin 18 develop chronic hepatitis, and have an increased susceptibility to drug-induced hepatotoxicity (2). Studies shows that high CK18 expression in tumor cells is associated with reduced invasiveness in vitro and lack of tumorigenicity in nude mice. It has been shown that high K18 expression correlated with a good prognosis and that reducing K18 expression increased the aggressiveness of established breast cancer cell lines (3).
Related Pathway
EGFR1 Signaling Pathway

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WB Image
Western blot - Cytokeratin 18 antibody; Anti-Cytokeratin 18 antibody at 1/50000 dilution + A431 cell lysate at 10 µg.Secondary.goat anti-rabbit HR at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 48 kDa.
WB Image
Western blot - Cytokeratin 18 antibody; Anti-Cytokeratin 18 antibody at 1/250 dilution + Human Cytokeratin 18 full length protein at 0.01 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 1 minute
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody; TA300825 at 1/250 dilution staining Cytokeratin 18 in urothelial carcinoma cells by Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody; TA300825 at 1/250 dilution staining Cytokeratin 18 in HeLa cells by Immunofluorescence

 

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