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Home Antibody All anti-KRT18 antibodies

Anti-KRT18 Antibody E431-1

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300412
  • Rabbit Monoclonal Antibody against KRT18 (Clone E431-1)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400083) , 20ug
100ul 325 In Stock
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WB(1)
IHC(8)
IF(1)
FC(1)
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Also for KRT18 (NM_000224)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues of human Cytokeratin 18 was used as immunogen.
Clone NameE431-1 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100; FC: 1:20; IHC-Fr: Use at an assay dependent dilution
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens keratin 18 (KRT18), transcript variant 1
Alternative NameCYK18; K18
Database LinkNP_000215
FunctionKeratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate eptithelial cells, forming alphahelical coiled-coil dimers which associate laterally and end to end to form 10-nm diameter filaments (4).
Related Pathway
EGFR1 Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cytokeratin 18 antibody [E431-1]; Anti-Cytokeratin 18 antibody [E431-1] at 1/10000 dilution + A431 cell lysate.Predicted band size : 48 kDa.Observed band size : 48 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody [E431-1]; Ab32118, at a dilution of 1/100, staining Cytokeratin 18 in paraffin embedded colon carcinoma tissue sections by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Gastric adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Normal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing negative staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing negative staining in Normal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); Fluorescent immunohistochemical analysis of paraffin-embedded human normal kidney tissue using TA300412. Green-CK18 red-PI.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [E431-1]; ICC/IF image of TA300412 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
FC Image
Flow Cytometry-Anti-Cytokeratin 18 antibody [E431-1](TA300412); Overlay histogram showing MCF7 cells stained with TA300412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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