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Home Antibody All anti-KRT18 antibodies

Anti-KRT18 Antibody E431-1

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300412 Rabbit Monoclonal Antibody against KRT18 (Clone E431-1) 100ul $325 In Stock
LC400083 KRT18 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(8)
IF(1)
FC(1)
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Also for KRT18 (NM_000224)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues of human Cytokeratin 18 was used as immunogen.
Clone NameE431-1 IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100; FC: 1:20; IHC-Fr: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens keratin 18, type I (KRT18), transcript variant 1
Alternative NameCYK18; K18
Database LinkNP_000215
Entrez Gene 3875 Human
FunctionKeratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate eptithelial cells, forming alphahelical coiled-coil dimers which associate laterally and end to end to form 10-nm diameter filaments (4).
Related Pathway Pathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Cytokeratin 18 antibody [E431-1]; Anti-Cytokeratin 18 antibody [E431-1] at 1/10000 dilution + A431 cell lysate.Predicted band size : 48 kDa.Observed band size : 48 kDa.
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Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 18 antibody [E431-1]; Ab32118, at a dilution of 1/100, staining Cytokeratin 18 in paraffin embedded colon carcinoma tissue sections by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Breast carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Gastric adenocarcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Normal colon tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing positive staining in Normal kidney tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing negative staining in Glioma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); TA300412 showing negative staining in Normal brain tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 18 antibody [E431-1](TA300412); Fluorescent immunohistochemical analysis of paraffin-embedded human normal kidney tissue using TA300412. Green-CK18 red-PI.
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ICC/IF image of TA300412 stained MCF7 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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Flow Cytometry-Anti-Cytokeratin 18 antibody(TA300412); Overlay histogram showing MCF7 cells stained with TA300412 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in MCF7 cells under the same conditions.
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