Invalid object name 'antibody_LocusMapping'. Anti-KRT15 Antibody, EPR1614Y - OriGene
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Anti-KRT15 Antibody EPR1614Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303573
  • Rabbit monoclonal antibody against Cytokeratin 15 (C-term) (EPR1614Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419427) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(3)
IF(1)
FC(1)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human CK-15 was used as an immunogen.
Clone NameEPR1614Y IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsICC/IF: 1:50 - 1:100; WB: 1:10000; FC: 1:100; IHC-P: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens keratin 15 (KRT15)
Alternative NameCK15; K15; K1CO
Database LinkNP_002266
FunctionThe protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cytokeratin 15 antibody [EPR1614Y]; Anti-Cytokeratin 15 antibody [EPR1614Y] at 1/10000 dilution + fetal thymus lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
Assay Image
Other - Anti-Cytokeratin 15 antibody [EPR1614Y]; Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 15 antibody [EPR1614Y]; TA303573 at 1/100 dilution staining Cytokeratin 15 in human squamous cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 15 antibody [EPR1614Y](TA303573); Fluorescent immunohistochemical analysis of paraffin-embedded human normal skin tissue using TA303573. Green-CK15 red-PI.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 15 antibody [EPR1614Y](TA303573); Fluorescent immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using TA303573. Green-CK15 red-PI.
IF Image
Immunocytochemistry/ Immunofluorescence - Cytokeratin 15 antibody [EPR1614Y]; TA303573 at 1/50 dilution staining Cytokeratin 15 in HeLa cells by Immunocytochemistry.
FC Image
Flow Cytometry - Anti-Cytokeratin 15 antibody [EPR1614Y]; Overlay histogram showing A431 cells stained with TA303573 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was was rabbit IgG (monoclonal) ( 1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

 

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