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Anti-KHDRBS1 Antibody EPR3232
Also for KHDRBS1 (NM_006559)
|A synthetic peptide corresponding to residues in human SAM68 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens KH domain containing, RNA binding, signal transduction associated 1 (KHDRBS1), transcript variant 1|
|p62; p68; Sam68|
|SAM68 (Src-associated protein in mitosis 68 kDa) is a member of the signal transduction and activation of RNA family of KH domain-containing RNA binding proteins (1). It is a major substrate of the Src tyrosine kinase in mitotic cells, having a tyrosine-rich C-terminal region and six polyproline (SH3-binding) sites, many of which are located in an amino-terminal region. Once phosphorylated, SAM68 functions as an adaptor protein in signal transduction cascades by binding to SH2- and SH3-domain containing proteins (2). Tyrosine phosphorylation also negatively regulates the nucleic acid binding properties of SAM68 (3).|
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Western blot - SAM68 antibody [EPR3232]; All lanes : Anti-SAM68 antibody [EPR3232] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lane 4 : A673 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 48 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAM68 antibody [EPR3232]; ab109197, at 1/250, staining SAM68 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - SAM68 antibody [EPR3232]; ab109197, at 1/250, staining SAM68 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-SAM68 antibody [EPR3232]; Overlay histogram showing HeLa cells stained with ab109197 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.