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Anti-KDR PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1214 (F-H-YP-D-N).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|VEGFR2 (Phospho-Tyr1214) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1214.
|Homo sapiens kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR)|
|CD309; FLK1; VEGFR; VEGFR2|
Cytokine-cytokine receptor interaction
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Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using VEGFR2 (Phospho-Tyr1214) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded breast carcinoma, using VEGFR2 (Phospho-Tyr1214) Antibody.The picture on the right is treated with the synthesized peptide.