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Anti-KDR Phospho Antibody
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|The antiserum was produced against synthesized phosphopeptide derived from humanVEGFR2 around the phosphorylation site of tyrosine 951 (K-D-YP-V-G).|
|Human, Mouse, Rat
||WB: 1:500~1:1000, IHC: 1:50~1:100
|phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. (Protein A or G Sepharose)
|Homo sapiens kinase insert domain receptor (a type III receptor tyrosine kinase) (KDR)|
|CD309; FLK1; VEGFR; VEGFR2|
Cytokine-cytokine receptor interaction
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Western blot analysis of extracts from SK-OV3 cells using VEGFR2 antibody (Lanes 1 and 2) and VEGRF2 (phospho-Tyr951) antibody (Lanes 3 and 4).
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using VEGRF2 (phospho-Tyr951) antibody.