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Anti-KCNJ3 TRUEMAB Antibody Clone 3E11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for KCNJ3 (NM_002239)
|Human recombinant protein fragment corresponding to amino acids 279-501 of human KCNJ3(NP_002230) produced in E.coli.|
|Human , Monkey , Mouse
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 3 (KCNJ3), transcript variant 1|
|GIRK1; KGA; KIR3.1|
|Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCNJ3 (RC205322, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCNJ3.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KCNJ3 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504152)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KCNJ3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA504152)
Anti-KCNJ3 mouse monoclonal antibody (TA504152) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCNJ3(RC205322).
HEK293T cells transfected with either RC205322 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KCNJ3 antibody(TA504152), and then analyzed by flow cytometry.