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Anti-JUN TRUEMAB Antibody Clone OTI1B10
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human JUN (NP_002219) produced in E.coli.|
|| WB 1:2000, IHC 1:500,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JUN (RC209804, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JUN.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-JUN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-JUN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800596)