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Home Antibody All anti-JAK3 antibodies

Anti-JAK3 Antibody EP909Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303561
  • Rabbit monoclonal antibody against JAK3(EP909Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424859) , 20ug
100ul 325 3-7 Days
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WB(1)
FC(1)
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Also for JAK3 (NM_000215)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human JAK3 was used as an immunogen.
Clone NameEP909Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:2000; FC: 1:100; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens Janus kinase 3 (JAK3)
Alternative NameJAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK
Database LinkNP_000206
FunctionThe protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq].
Related Pathway
Jak-STAT signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - JAK3 antibody [EP909Y]; Anti-JAK3 antibody [EP909Y] at 1/2000 dilution + TF-1 cell lysate.Predicted band size : 125 kDa.Observed band size : 125 kDa.
FC Image
Flow Cytometry - Anti-JAK3 antibody [EP909Y]; Overlay histogram showing Jurkat cells stained with TA303561 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1ug/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

 

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