The antiserum was produced against synthesized non-phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 570 (G-D-YP-G-Q).
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Concentration
1mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
Guaranteed Application *
WB, IF
Suggested Dilutions
WB: 1:500~1:3000, IF: 1:100~1:500, ELISA: 1:5000
Background
JAK2 (Ab-570) antibody detects endogenous levels of total JAK2 protein.
Related Pathway
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
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Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 24hours), using JAK2 (Ab-570) antibody.The lane on the right is treated with the synthesized peptide.
