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Home Antibody All anti-JAK2 antibodies

Anti-JAK2 PHOSPHO Antibody E132

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA303559
  • Rabbit monoclonal antibody against Phospho-Jak2 (pY1007/1008)(E132) (phospho-specific)
100ul $325 2 Weeks
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WB(3)
IHC(6)
DB(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen. The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.
Clone NameE132 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, DB Suggested DilutionsWB: 1:1000 - 1:10000; IHC-Fr: Use at an assay dependent dilution; IHC-P: Use at an assay dependent dilution; ICC: 1:50 - 1:100; IP: 1:50; Dot Blot: 1:1000
Predicted MW Explanation 130 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens Janus kinase 2 (JAK2)
Alternative NameJTK10; THCYT3
Database LinkNP_004963
Entrez Gene 3717 Human
Entrez Gene 16452 Mouse
Entrez Gene 24514 Rat
FunctionThis gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome Chemokine signaling pathwayJak-STAT signaling pathwayAdipocytokine signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - JAK2 (phospho Y1007 + Y1008) antibody [E132]; Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] at 1/50 dilution + immunoprecipitation pellets from Jurkat cell lysate.Predicted band size : 130 kDa.Observed band size : 120 kDa .
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Western blot - JAK2 (phospho Y1007 + Y1008) antibody [E132]; All lanes : Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] at 1/2000 dilution.Lane 1 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 0 hours.Lane 2 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 15 minutes.Lane 3 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 30 minutes.Lane 4 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 1 hour.Lane 5 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 2 hours.Lane 6 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 4 hours.Lane 7 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 6 hours.Lane 8 : Rat uterine cell line - whole cell lysate. Treated with 1ug/mL Prolactin for 24 hours.Lysates/proteins at 30 ug per lane.Secondary.An HRP-conjugated donkey anti-rabbit polyclonal. at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 130 kDa.Observed band size : 110 kDa .Additional bands at : 55 kDa (possible non-specific binding).
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Figure from citation: Western Blot of JAK2 protein level by using anti-JAK2 antibody in mouse Ba/F3 cells. View Citation
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Immunohistochemistry (Paraffin-embedded sections) - JAK2 (phospho Y1007 + Y1008) antibody [E132]; TA303559 staining JAK-2 on human lung adenocarcinoma in IHC-P.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132]; TA303559 showing positive staining in Lung adenocarcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132](TA303559); TA303559 showing positive staining in Ovarian carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132](TA303559); TA303559 showing positive staining in Cervical carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132](TA303559); TA303559 showing positive staining in Endometrial carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132]; TA303559 showing positive staining in Urinary bladder transitional carcinoma tissue.
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Dot Blot - JAK2 (phospho Y1007 + Y1008) antibody [E132]; Analysis of immunogen phosphopeptide (A) and non phosphopeptide (B). Antibody used at 1/1000 dilution.
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