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Anti-JAK2 PHOSPHO Antibody E132

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA303559
  • Rabbit monoclonal antibody against Phospho-Jak2 (pY1007/1008)(E132) (phospho-specific)
100ul 325 In Stock
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WB(2)
IHC(6)
DB(1)
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Also for JAK2 (NM_004972)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and Tyr1008 of human JAK2 was used as immunogen. The antibody only detects JAK2 phosphorylated on Tyrosine 1007 and 1008.
Clone NameE132 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, DB Suggested DilutionsWB: 1:1000 - 1:10000; IHC-Fr: Use at an assay dependent dilution; IHC-P: Use at an assay dependent dilution; ICC: 1:50 - 1:100; IP: 1:50; Dot Blot: 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens Janus kinase 2 (JAK2)
Alternative NameJTK10; THCYT3
Database LinkNP_004963
FunctionThis gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq].
Related Pathway
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Jak-STAT signaling pathway

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WB Image
Western blot - JAK2 (phospho Y1007 + Y1008) antibody [E132]; Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] at 1/50 dilution + immunoprecipitation pellets from Jurkat cell lysate.Predicted band size : 130 kDa.Observed band size : 120 kDa .
WB Image
Western blot - JAK2 (phospho Y1007 + Y1008) antibody [E132]; All lanes : Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] at 1/2000 dilution.Lane 1 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 0 hours.Lane 2 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 15 minutes.Lane 3 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 30 minutes.Lane 4 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 1 hour.Lane 5 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 2 hours.Lane 6 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 4 hours.Lane 7 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 6 hours.Lane 8 : Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 24 hours.Lysates/proteins at 30 µg per lane.Secondary.An HRP-conjugated donkey anti-rabbit polyclonal. at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 130 kDa.Observed band size : 110 kDa .Additional bands at : 55 kDa (possible non-specific binding).This image is courtesy of an anonymous Abreview
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - JAK2 (phospho Y1007 + Y1008) antibody [E132]; TA303559 staining JAK-2 on human lung adenocarcinoma in IHC-P.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132]; TA303559 showing positive staining in Lung adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132](TA303559); TA303559 showing positive staining in Ovarian carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132](TA303559); TA303559 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132](TA303559); TA303559 showing positive staining in Endometrial carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132]; TA303559 showing positive staining in Urinary bladder transitional carcinoma tissue.
DB Image
Dot Blot - JAK2 (phospho Y1007 + Y1008) antibody [E132]; Analysis of immunogen phosphopeptide (A) and non phosphopeptide (B). Antibody used at 1/1000 dilution.

 

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