Home Antibody All anti-JAG1 antibodies
Also for JAG1 (NM_000214)
|This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-125 of human Jagged-1protein.|
|WB, IHC, IF
||ELISA: 1:20,000 - 1:100,000, WB: 1:500 - 1:2,000, IHC: 1:100 - 1:500, IF: 1:200 - 1:1,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Anti Jagged 1 Antibody recognizes the jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.
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Western blot using Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 µg of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.
Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used. Personal Communication. Martin Kast Laboratory.
Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.