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Anti-Il17a Antibody Polyclonal
Also for Il17a (NM_010552)
|E.coli derived Recombinant Murine IL-17 (IL-17A) |
||Lot dependent; please refer to CoA along with shipment
|A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-17A (murine IL-17A). Anti-Murine IL-17A specific antibody was purified by affinity chromatography employing immobilized mIL-17A matrix.
|Mus musculus interleukin 17A (Il17a)|
|Ctla-8; Ctla8; IL-17; IL-17A; Il17|
|Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.|
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To detect mIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-17A is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mIL-17A by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine IL-17A (61-017ABT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-17A.