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Anti-ITGAX Antibody EP1347Y
Also for ITGAX (NM_000887)
|A synthetic peptide corresponding to residues on the C-terminus of human ITAX was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50,000 100,000; IHC: 1:100 250; ICC: 1:50 100; IP: 1:80
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), transcript variant 2|
Entrez Gene 3687 Human
|This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. [provided by RefSeq]. |
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Anti-CD11c antibody [EP1347Y] (TA303556) at 1/1000 dilution + THP-1 + TPA lysate at 10 ug. Secondary: goat anti-rabbit HRP at 1/2000 dilution
Human paraffin-embedded lymph node stained with TA303556 at 1/100 - 1/250 dilution
TA303556 at 1/400 dilution staining CD11c in human tonsil tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, prior to blocking in 10% BSA in FCS (20ml) + DMEM (80ml) for 30 minutes at 21°C and then incubated with TA303556 for 20 hours at 4°C. A biotin conjugated rabbit polyclonal to mouse Ig, diluted 1/400, was used as the secondary antibody.
TA303556 showing positive staining in Normal tonsil tissue.
TA303556 showing negative staining in Normal brain tissue.
TA303556 showing negative staining in Normal breast tissue.
TA303556 showing negative staining in Skeletal muscle tissue.
TA303556 showing positive staining in Normal spleen tissue.